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Visualising the Vasculature of a Human Glioma in Mouse Brain using CLARITY

Liu King Lun, Justyna Prystal, Nelofer Syed* and Hei Ming Lai
Division of Brain Sciences, Department of Medicine, Imperial College London, Hammersmith Hospital Campus, United Kingdom
*Corresponding Author: Nelofer Syed, Department of Medicine, Imperial College London, Hammersmith Hospital Campus, Burlington Danes Building, Rm E506, London W12 0NN, UK, Tel: 44 (0)20 7594 5292, E-mail: n.syed@imperial.ac.uk
Received: February 20, 2016; Accepted: February 23, 2016; Published: February 26, 2016
Citation: Lun LK, Prystal J, Syed N, et al. Visualising the Vasculature of a Human Glioma in Mouse Brain using CLARITY. Arch Cancer Res. 2016, 4:1.
 
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Abstract

Advancement of laser microscopy and molecular probing techniques in recent years has led to the development of various tissue clearing technologies to allow us to visualise a large piece of tissue in 3D. Using an adaptation of a novel tissue clearing technique, CLARITY, we were able to visualise vascularisation of tumour in a completely new dimension. This image is a colourcoded depth projection (z-stack depth = 618.76 m) of a piece of clarified mouse brain tissue with human glioma immunostained with CD31 antibodies, showing large penetrating branches of blood vessels at the tumour margin

 

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